EasyPrep Genomic DNA Extraction Kit
EasyPrep Genomic DNA Kit is based on silica membrane technology and special buffer system for many kinds of sample’s gDNA extraction. The spin column made of new type silica membrane can bind DNA optimally on given salt and pH conditions. Simple centrifugation processing completely removes contaminants and enzyme inhibitors such as proteins and divalent cations. Purified DNA is eluted in low-salt buffer or water, ready for use in downstream applications. DNA purified by EasyPrep Genomic DNA Kit is highly suited for restriction analysis, PCR analysis, Southern blotting, and cDNA library.
Features
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Suitable for blood, cell, and animal tissues
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No need to use phenol/chloroform
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High yield and purity gDNA
Yeild of Genomic DNA with EasyPrep Genomic DNA Kit
| Source | DNA Yeild |
| Whole blood from mammalian (100 µ l- 400 µ l) | 3- 10 µ g |
| Whole blood from birf or amphibian (5 µ l- 20 µ l) | 5- 40 µ g |
| Cultured cells (10 6-107 cells) | 5- 30 µ g |
| Tissue (30 mg) | 10- 30 µ g |
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Workflow
DNA extracted from EasyPrep Genomic DNA Extraction Kit:
Genomic DNA extracted from long-stored
blood samples using EasyPrep Genomic
DNA Extraction Kit. 3ul of 100ul elutes were
loaded per lane.
- Huang YH et al. "Heat Shock Protein 60 Restricts Release of Mitochondrial dsRNA to Suppress Hepatic Inflammation and Ameliorate Non-Alcoholic Fatty Liver Disease in Mice." Int J Mol Sci. 2022 Jan 5;23(1):577.
- Yang CH et al. "A Novel Murine Model Expressing a Chimeric mSCARB2/hSCARB2 Receptor Is Highly Susceptible to Oral Infection with Clinical Isolates of Enterovirus 71." J Virol. (2019) May 15;93(11):e00183-19. PMC6532076.
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Chang CCh et al. "The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis." Scientific Reports 8.1 (2018): 9786.
Q1. Can this product be stored and shipped under -80°C
A: No, it is not suitable. This kit contains columns made of silica membrane and the DNA binding efficiency of the membrane will be affected with damp if you store and ship this kit under -80°C
Q2. Can EasyPrep Genomic DNA Extraction Kit (DPT-BC04) preserve 500bp DNA fragments?
A: Yes. Our membrane can preserve DNA fragments over 200bp
A: No, it is not suitable. This kit contains columns made of silica membrane and the DNA binding efficiency of the membrane will be affected with damp if you store and ship this kit under -80°C
Q2. Can EasyPrep Genomic DNA Extraction Kit (DPT-BC04) preserve 500bp DNA fragments?
A: Yes. Our membrane can preserve DNA fragments over 200bp